What is FPIA assay?
Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay, that consists of a simple prepare and read method, without the requirement of separation or washing steps.
What does FPIA mean?
fluorescent polarization immunoassay
, fluorescent polarization immunoassay Abbreviation: FPIA. A means of identifying and quantifying the amount of an antigen in a specimen in which a fluorescently labeled antibody is mixed with a sample thought to contain the antigen is exposed to polarized light.
What is fluoro enzyme immunoassay?
Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria.
What is the principle of fluorescence immunoassay?
A modern fluorescent based immunoassay uses as the detection reagent a fluorescent compound which absorbs light or energy (excitation energy) at a specific wavelength and then emits light or energy at a different wavelength.
How does emit work?
The enzyme-multiplied immunoassay technique (EMIT®) is a simple, rapid homogeneous method now commonly employed to measure a wide range of substances (particularly drugs). The technique works on the basis that the drug present is proportional to the inhibition of an enzyme substrate reaction.
How does Cedia immunoassay work?
A cloned enzyme donor immunoassay (CEDIA) is a competitive homogenous enzyme immunoassay. This assay makes use of two component fragments of an enzyme which are each individually inactive. Competition for the antibody occurs between the analyte in the sample and the enzyme-fragment-analyte-conjugate.
How does an immunoassay work?
Immunoassays are based on the principles that specific antigens will stimulate very specific (unique) immune responses and that the proteins produced by the immune response, called antibodies, can be used to signal the presence of a target compound in a sample.
What is the principle of enzyme immunoassay?
ELISA is also known as a solid-phase enzyme immunoassay that is used to detect the presence of a specific protein (antigen or antibody) in blood samples. The basic principle of ELISA is to use an enzyme to detect the binding of antigen (Ag) or antibody (Ab).
What type of immunoassay is EMIT?
The enzyme-multiplied immunoassay technique (EMIT) is the most widely used homogeneous enzyme-labeled immunoassay, particularly for the detection of small drugs. EMIT was developed by Rubenstein et al. (1972).
What is the EMIT test used for?
Enzyme multiplied immunoassay technique (EMIT) is a common method for qualitative and quantitative determination of therapeutic and recreational drugs and certain proteins in serum and urine.
What is Cedia in drug testing?
The CEDIA method is a single phase, competitive binding process that combines analyte-specific antibodies and two genetically engineered fragments of the bacterial enzyme ß-galactosidase to accurately and reliably detect the presence of drugs and their metabolites as well as other substances in serum, plasma, whole …