What does beta-mercaptoethanol do in cell culture?
Gibco® 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation.
What is beta-mercaptoethanol used for?
β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
What is the concentration of beta-mercaptoethanol?
When preparing SDS-PAGE sample buffer, you can use either beta-mercaptoethanol (BME) or dithiothreitol (DTT). For BME, use a concentration of 5% (about 100 mM).
What is the molarity of beta-mercaptoethanol?
The sigma Beta Mercaptoethanol which we use in our lab is always 14.3M.
Is beta-mercaptoethanol toxic to cells?
2-mercaptoethanol or beta mercaptoethanol is a reducing agent, thus when added to a cell line, it reduces toxic oxygen radicals.
What beta-mercaptoethanol tells us?
Beta-mercaptoethanol (ß-ME) is a reducing agent that will irreversibly denature RNases by reducing disulfide bonds and destroying the native conformation required for enzyme functionality.
Is beta-mercaptoethanol toxic?
BME can be toxic if ingested, and fatal if inhaled or absorbed through the skin. Vapors can irritate the eyes, mucous membranes, and respiratory tract. Symptoms of inhalation exposure may include coughing, sore throat, and/or shortness of breath. BME is combustible as a liquid or vapor!
Is 2-mercaptoethanol acidic or basic?
2-Mercaptoethanol
| Names | |
|---|---|
| log P | -0.23 |
| Vapor pressure | 0.76 hPa (at 20 °C) 4.67 hPa (at 40 °C) |
| Acidity (pKa) | 9.643 |
| Basicity (pKb) | 4.354 |
Is beta mercaptoethanol carcinogenic?
Causes skin irritation and may be absorbed in the body in toxic quantities. Vapors irritate the eyes with redness and pain. Splashes may cause severe irritation. Substance is neither a known nor an anticipated carcinogen.
Why is beta mercaptoethanol toxic?
Vapors can irritate the eyes, mucous membranes, and respiratory tract. Symptoms of inhalation exposure may include coughing, sore throat, and/or shortness of breath. When BME is heated to decomposition, toxic fumes including sulfur oxides and carbon oxides will be emitted. BME is combustible as a liquid or vapor!
How is GIBCO 2-mercaptoethanol used in cell culture?
Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation. Gibco 2-Mercaptoethanol contains 2-mercaptoethanol at a concentration…
What is the concentration of 2-mercaptoethanol in DPBS?
2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation. Gibco 2-Mercaptoethanol contains 2-mercaptoethanol at a concentration of 55 mM in Dulbecco’s phosphate buffered saline (DPBS).
When to add 2 mercaptoethanol to medium?
The 2-Mercaptoethanol must be added to the medium immediately before use because activity rapidly declines in diluted form. You can supplement enough medium needed for that day. No results found for your search criteria.
Where is GIBCO 2-mercaptoethanol manufactured in the USA?
Gibco 2-Mercaptoethanol contains 2-mercaptoethanol at a concentration of 55 mM in Dulbecco’s phosphate buffered saline (DPBS). Gibco Reducing Agent is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified…